FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21199609
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATAT20450.015.3985321
GCGAAAT28050.014.7450971
GTTTTTT259700.014.3153631
CGAATCG17200.013.9360473
CGAAAAT44600.013.6995521
CTCGATT36400.013.6868141
CGCGAAT13200.013.1742431
AAATCCA187200.012.6789544
CGCAAAC41200.012.6626221
CGAAAAC32000.012.043751
AATCCAA102050.012.0205775
AAAAATC198850.011.7943172
GTTTAAT79300.011.7351841
CGCAAAT27850.011.6445251
GGAAAAT166700.011.6442711
TCGATTG20200.011.40099052
CTCGGAT64150.011.282931
CGCGTTT15950.011.1974931
GCCGAAT44100.011.1904751
CGGAAAT43050.011.1358881
Produced by FastQC (version 0.11.5)