FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22647988
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG80200.046.70697894
CAAGTCG62400.026.21153694
CGCGTAA7500.023.1866651
TAAGTCG26850.021.53072494
GAAGTCG103950.016.27705694
GTCGGTT64850.015.9444871
GTTTATT118050.014.9301131
GTTCGAT36400.014.0741751
AATCGGG68150.013.5172415
GTTTGAT138050.013.2777981
CAATCGG40050.013.1435694
TTAGCGA32250.013.1162797
AAAGTCG38400.013.096354594
TAGCGAG29950.012.8681138
GCGTAAC14250.012.5333332
CGCGGGT68300.012.5241571
GATTGTA64000.012.4843744
GTTTTCT245350.012.2600361
GTTTTAT181750.012.2574961
CGGAAAT43800.012.1255691
Produced by FastQC (version 0.11.5)