FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22647988
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG105000.036.4809594
CAAGTCG82100.021.81120594
TAAGTCG25500.021.7490294
CGCGTAA6650.017.669171
GAAGTCG97350.016.70467494
GTCGTTT21100.013.5876761
GTCGGGT41700.013.4124691
CGCAACT32300.013.3869961
AAAGTCG46300.013.29805794
GTTTATT122700.013.2534631
GTAAAAC62500.013.161
GTCGGTT58400.012.7962321
CGCAATT20800.012.6538471
TATACGC8350.012.3832337
AATCGTA19000.012.3684215
TCGGGTC46000.012.3630432
CGAATCG14300.012.1608383
ATATACG10450.012.1435396
CGCAAAC42400.012.0825471
CAATCGG38000.011.8736844
Produced by FastQC (version 0.11.5)