FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23190687
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG114750.039.03355494
CAAGTCG90650.020.6872694
TAAGTCG29150.017.7358594
GAAGTCG110700.017.61969494
CGCGTAA8250.015.3818191
GTTCGAT29450.012.2886261
TTAGCGA23050.011.8264668
GTCCGAT14600.010.6232881
CGTATCG19850.010.4181373
CTCGGTT35900.010.21171
TTCGATT31800.010.1981142
GTCGTTT22650.010.1677711
TAGCGAG29750.010.1109259
GGCGGTT55950.09.9964261
CTCGGAT60600.09.8498361
AATCGTA21500.09.8372115
AAAGTCG46850.09.83137794
GTCGGTT50900.09.7878191
CGCGTTT15400.09.7662341
CGGAAAT45600.09.6885971
Produced by FastQC (version 0.11.5)