FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18534379
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG57850.036.88504894
TAAGTCG20250.017.87160594
CAAGTCG46700.017.10920794
GTTTTTT284500.016.487171
CGCAAAT26600.015.1954871
TTTTTTC385050.014.2080242
TATACGC7250.013.6137937
TTTTTTG428950.013.5318792
GTTTAAT92200.013.2537961
TACGCTA7300.012.8767119
CTTAGAT59800.012.7324411
CGAAAAC28500.012.6982441
TCGATCC18200.012.6538462
CTCGGAT48500.012.5979381
TTTTTTA405650.012.5016632
GTTTTAC69100.012.3791591
GAAGTCG73800.012.29132794
GTTTTTC161900.012.2798011
GTTTTAT157850.012.2673431
AAATCGA29250.012.2119664
Produced by FastQC (version 0.11.5)