FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23665790
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG123550.056.2630594
TAAGTCG34250.029.3664294
CAAGTCG97850.028.91568894
GAAGTCG139500.023.01146994
AAAGTCG52050.015.62151794
CGAAAAT36600.015.4098361
GTTTTTT322350.015.2511231
TTTTTTC397100.013.2916142
CTCGATT44800.012.90401651
GTTTAAT96300.012.3966761
TTTTTTG438550.011.9174552
TACGCTA8350.011.8203589
CGCGGAT25350.011.4950691
GTTTTCT231000.011.4142861
TATACGC9550.011.319377
AAATCCA207650.011.2944854
GGAAAAT188500.011.2450921
GTTTTAT170250.011.1806151
GTTTTTC177850.011.099241
GAAAAAT233650.011.0434411
Produced by FastQC (version 0.11.5)