FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21019115
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG133550.060.88356894
CAAGTCG109700.030.59070294
GAAGTCG127900.025.90695894
TAAGTCG39000.025.06666694
AAAGTCG52400.019.28435194
GCGAAAT29550.019.0862941
TTTTTTC406200.016.8121622
CGAAATA20500.016.5073182
GTTTTTT298900.016.337571
GTTTTAC62000.015.6161281
TCGGATC43050.015.2845532
CTCGGAT67700.014.3013291
CGAATCG19250.014.1610393
GTCAATT54850.014.0528721
AATCGTA23950.013.9331945
GTTTTTC159100.013.7661851
TCGAATC15350.013.4723132
AAATCCA188500.013.3893914
AAAAATC188150.013.38931752
GTCGATT21200.013.3018871
Produced by FastQC (version 0.11.5)