FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21019115
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG184950.044.31900894
CAAGTCG138850.025.65790494
TAAGTCG40150.023.17808394
GAAGTCG155250.019.98067794
GTTTTTT276000.016.5351471
CGCGAAT12250.016.497961
AAAGTCG68700.015.803493594
GCGAAAT33200.015.4307241
AAAAATC190550.014.6266072
GCGGAAT61200.014.2843131
GAAAAAT194900.014.1072341
AAATCCA171350.013.8243364
GCCGAAT48000.013.1208331
CGAAAAC30450.013.1198681
AAAATCC165000.012.8181833
AATCCAC100250.012.6114725
CGCGATT6351.6007107E-1012.5826771
CTCGATT51200.012.5761721
TCGATTG37250.012.4912752
AATCGGG81700.012.4834775
Produced by FastQC (version 0.11.5)