FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22217360
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG107900.049.74420594
CAAGTCG86600.024.80253894
TAAGTCG32750.022.9618394
GAAGTCG124750.018.34789594
AAAGTCG44500.016.47640494
GTTTTTT315700.013.4583461
CGAAAAT32700.013.223241
TTTTTTC405400.012.0572262
GTTTTAC69500.011.8345321
GTCGGTT46550.011.4092371
CGGAAAT43350.011.1672431
CGCAAAT27600.011.068841
GTTTAAT91250.010.8164381
GGAAAAT180650.010.7971211
GTTTTAT161000.010.6260861
AATCGTA21250.010.6164695
GCGAAAT27100.010.5793351
TTTTTTG430750.010.5729532
CGAAGAT88250.010.5450411
GTTTATT117900.010.4045791
Produced by FastQC (version 0.11.5)