FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22288497
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG180800.063.35122394
CAAGTCG130150.033.9815694
TAAGTCG44950.030.42714394
GAAGTCG180600.027.50775394
AAAGTCG57150.020.72441194
TATACGG21650.016.9330278
TAGCGTA9250.016.7675694
GTATACG23350.015.90157
GCGTATA12400.013.26612956
ATAAGTC51550.012.12609294
CGTATAT17150.011.7842577
ACAAGTC130900.011.48968694
ATAGCGT15550.010.2765273
AATAGCG16050.010.2492222
GCAAGTC130000.010.23153994
AGAAGTC255950.09.56710394
TTAAGTC64300.09.35614494
CGAAGTC90250.09.32188494
TATATCG9106.0845196E-99.2967037
CGTATCG19400.08.60051636-37
Produced by FastQC (version 0.11.5)