FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22288497
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT276050.014.76145651
CGGATAT18700.014.3262031
CGAAAAC35850.014.0278951
CGCAAAC41650.013.8799521
GTTTTAT157150.013.54820251
CGAAAAT42750.013.5228081
CTTTAAC69650.013.4960531
CTCGATT38550.013.2892371
CGCAAAT30850.012.7974071
CTTAGAT54400.012.5275741
CGCAATC21450.012.48951151
CTCAGAT179950.012.4584621
GGAAAAT164300.012.3292761
CTCGTTT40500.012.3012351
AAAAATC198800.012.2701222
CGGAAAT46750.012.0641721
GTTTTTC151950.011.9394551
AAATCCA201850.011.8285864
GTTTAAT81750.011.6709491
GGAAAAC150150.011.4252421
Produced by FastQC (version 0.11.5)