FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22694094
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG105550.055.74988694
CAAGTCG92950.030.54115194
TAAGTCG28650.023.13089294
GAAGTCG118750.018.0084294
AAAGTCG45800.013.64847294
GTTCGAT28750.013.2417391
TTAGCGA23650.012.1226228
GTCGGTT48050.011.7377741
TAGCGAG30550.011.6923089
GTCGTTT21550.011.5591661
GTCCGAT15750.011.3396831
CGCGGAT24550.011.1038691
AATCGTA24750.011.0141425
GTCGATT23350.010.869381
GCGGTAT23450.010.8230291
GTCGGAT26100.010.8045981
TTCGATT31300.010.6613432
GTCGAAT14550.010.6597951
GCCGGTT53550.010.5322131
CTCGGTT36350.010.4731781
Produced by FastQC (version 0.11.5)