FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22694094
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG125850.046.6078794
CAAGTCG106100.028.26201894
TAAGTCG30150.025.09784394
GAAGTCG115500.017.8640794
GTTCGAT29450.014.2037351
TTAGCGA23750.013.4568438
TAGCGAG28200.012.3333339
AATCGTA22850.012.1356685
CGCGGAT25550.011.4050881
TTCGATT32650.011.37212852
GTCCGAT15950.010.9028221
CGCTAAT11350.010.76652051
CGCGGTT41450.010.5452371
GGCGATT41950.010.4195481
CTCGTTT35400.010.3559331
TCGGATT26950.010.2894262
GGCGGAT91500.010.2732251
CGGAAAT47800.010.2259421
CGAATCG13350.010.2097383
GGCGCTT106600.010.0525341
Produced by FastQC (version 0.11.5)