FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22342816
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG100100.050.098994
TAAGTCG29450.026.17317694
CAAGTCG77800.024.70822594
GAAGTCG121800.018.71510794
GTTTTTT302850.015.2243681
TTTTTTC384850.014.9481612
AAAGTCG46700.013.58672394
CGCAAAT27850.013.1633751
CGAATCG17150.012.3323613
TTTTTTG416850.012.2446932
GCGAAAT30550.012.1538461
AAAAATC204000.012.1416662
CGAAAAT35450.011.93231
CGGATAT25150.011.773361
AATCCAC113600.011.7086275
GTTTTCT215350.011.6326911
GAAAAAT213350.011.6315921
GGAAAAT164500.011.5428571
TTAGCGA24850.011.1589537
AAATCCA189450.011.0646614
Produced by FastQC (version 0.11.5)