FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22342816
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG122950.041.05571494
CAAGTCG91700.023.98691694
TAAGTCG28650.019.35776794
GAAGTCG117400.018.4957494
CGCAAAC46400.013.370691
GTTTTTT285150.013.0541811
CGGATAT23150.012.3844491
CGAAAAT50500.012.0059411
AAAGTCG54350.011.84728694
AAAAATC201000.011.7850752
CGAAAAC35350.011.5671851
CGAAGAT79400.011.4836281
CGCGGAT25600.011.1992191
CGGAAAT46150.011.1007591
CTCGGAT67600.010.8461541
CTTAGAT53400.010.7378281
GTCGATT19700.010.7360411
TTAGCGA21950.010.706157
CGCAAAT28550.010.5359021
GGAAAAC137950.010.4255161
Produced by FastQC (version 0.11.5)