FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22148417
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG127050.039.6938294
CAAGTCG96300.023.96365594
TAAGTCG33150.019.7073994
GAAGTCG120750.016.77598494
GTCGGTT37000.013.7189191
AAAGTCG54250.013.16866494
TTTAGCG18200.012.9120872
CGGAAAT42350.012.5407321
CGCAAAC43450.012.4395851
CGCAAAT28350.012.4338621
TACGCTA7950.012.4150949
GGAAAAC148700.012.26361751
CGAATCG13100.012.1984733
TATACGC7904.3655746E-1111.3037977
GGCGAAT39100.011.29923251
CTCGGTT41750.011.0323351
AATCGGG31250.010.9791995
TTAGCGC19850.010.8916873
TCGGATT26400.010.8598482
GTTTTAC63750.010.8376471
Produced by FastQC (version 0.11.5)