FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23117291
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG87400.049.2048194
CAAGTCG71300.026.96073294
TAAGTCG29650.023.77740594
GAAGTCG112750.017.3410294
TCGGATT28550.011.8528912
GTTCGAT28250.011.6460191
CGCGAAT8401.0913936E-1111.1904761
AAAGTCG43350.011.05882494
GTCGATT21200.010.641511
GTCGTTT23050.010.6030381
GTCCGAT15950.010.313481
GTCGGTT51700.09.7272741
TTAGCGA24250.09.69072158
TAGCGAG29800.09.6208069
GTTTATT108150.09.6042541
TTCGATT35450.09.4132592
GGCGAAT47250.09.35026551
AATCGTA23300.09.2789715
CGGGAAT54450.09.1496791
CGTATCG18000.09.1388893
Produced by FastQC (version 0.11.5)