FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22044374
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG86400.049.17592294
CAAGTCG65900.024.1775494
TAAGTCG29950.020.24373894
GAAGTCG100900.018.91179394
TTTTTTC440600.016.1609172
TTAGCGA21450.015.99533757
GTTTTTT327350.015.2191831
CGAATCG16400.014.9024393
TTTTTTG505050.014.4522322
TTTTTTA493700.014.2132872
CGCAAAT30450.013.27421951
GGAAAAC166200.013.0084241
CGCATAT7950.013.0062891
TAGCGAG27200.012.95955858
GTTTTAC78650.012.9078191
TACGCTA6953.6379788E-1212.848929
GGAAAAT195400.012.7241541
AATCGTA21600.012.6203695
CGGAAAT47400.012.493671
GTTTTCT233250.012.3116821
Produced by FastQC (version 0.11.5)