FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21959898
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG64200.035.7990794
CAAGTCG52550.019.05042894
TAAGTCG20550.018.52554994
GTTTATT107600.016.7732351
CGGAAAT42600.014.5633811
GTCGGTT53500.013.9682251
GTTCGAT31300.013.6645371
CGCGGGT65900.013.2655541
GTCAATT59350.013.2249371
CGAAAAT36650.013.2087321
CAATCGG36800.013.0271744
GTTTAAT99900.012.6556571
AATCGGG58800.012.4693895
GTTTTAT160900.012.0932261
TAATCGG14200.011.9154934
GTTTTAC66450.011.741161
CGCAACT32700.011.6422011
GGAAAAT167150.011.6410421
CGAATCG12700.011.4724423
AAATCCA190950.011.4454054
Produced by FastQC (version 0.11.5)