FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305983

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305983
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22705043
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG104850.052.4463594
CAAGTCG87350.028.89410494
TAAGTCG29700.021.52188594
GAAGTCG121400.020.20922594
CGCGTAA9400.013.01
GTCGTTT19400.012.8402051
GCGGTAT25500.012.5333341
GTTCGAT31450.012.4038161
AAAGTCG43700.011.40045794
GTCCGAT13650.011.0183141
CTCGGTT37750.010.7072841
TTCGATT33100.010.6495472
GGCGAAT65350.010.6442241
TATACGG17050.010.61290334-35
CGGTATA14450.010.4083042
CGTTCGA17350.010.2939487
TTAGCGA19700.010.25888258
GTCGGAG40350.010.1338291
CGGAAAT46650.010.07502651
TCGGATT24800.010.0443552
Produced by FastQC (version 0.11.5)