FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22705043
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG128000.043.36484594
CAAGTCG101000.026.43168394
TAAGTCG29900.024.05016594
GAAGTCG121300.016.38994294
AAAGTCG51900.013.49325794
CGTATCG18050.012.4986163
GTTCGAT31350.012.2934611
TTAGCGA19600.011.758
GTCCGAT14800.011.751
TTCGATT34900.011.3123222
TATACGG16750.010.94328334-35
GTCGTTT20650.010.9249391
CGCGTAT4501.0605587E-410.4444451
GGCGGTT51450.010.3226441
TAGCGAG25050.010.1317379
GGGAAAT141400.010.1046671
GGCGAAT54000.010.0962961
CGCAATT22400.010.0714281
GCCGGTT53850.010.037141
CCGGTTA18000.09.9222222
Produced by FastQC (version 0.11.5)