FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305986

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305986
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22473058
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG146400.051.33402394
CAAGTCG122500.031.03918694
TAAGTCG35450.026.64880294
GAAGTCG127650.021.61300794
CGCGTAA6550.015.7862611
GTCCGAT16000.014.6875011
AAAGTCG53900.012.81818394
AATCGTA20350.012.70270255
TTAGCGA20750.011.5518088
TAGCGAG24750.011.393949
CGCGGAT25400.011.1023631
GTTCGAT25000.011.0920011
GTCGTAT10850.010.82949351
TCGGATT24050.010.7484412
GCCGTAT18200.010.3296721
CGCAAAT26200.010.2251921
ATCGTAT17150.010.1399426
CGGAAAT39500.010.1139251
CCGTATC28800.09.7916672
GCCGGTT55900.09.5849731
Produced by FastQC (version 0.11.5)