FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305988

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305988
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22294054
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG165400.049.52902694
CAAGTCG122550.029.76091694
TAAGTCG38350.023.40808394
GAAGTCG149500.020.18327994
GCGAAAT27250.016.9027541
GCCGAAT50050.016.2457561
AAAGTCG64850.015.14726394
CGAATCC49500.014.4323243
GTTTTTT268000.013.2231351
AAAAATC188850.012.8917142
CGAAAAC38700.012.7519391
CGCAAAC45800.012.6222721
AAATCCA192050.012.6034894
CGAAGAT80600.012.5955341
GAAAAAT194400.012.2093621
CTTAGAT52100.012.1785031
CCGAATC75200.012.0000012
AATCCAC114150.011.9816045
TCGAATC17850.011.8487412
CGCAAAT28250.011.8123891
Produced by FastQC (version 0.11.5)