FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22918080
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG85700.046.94515694
CAAGTCG68200.024.4648194
TAAGTCG28650.022.14659794
GAAGTCG107050.017.1228494
GTTCGAT26950.014.8237491
GTCCGAT17100.014.5672521
TTAGCGA22800.013.6052648
GTCGGTT47500.012.1705271
TAGCGAG31300.012.1629399
TCGGATT28850.011.8925492
CGCGTAT4355.7632788E-611.8850581
CGTCGGA28400.011.58450894
AATCGTA23650.011.3276965
AAAGTCG46250.011.2894
CGACCTA10100.011.1683184
TTCGATT35000.011.011432
CGCTTAA38900.010.93444744-45
TATACGG18900.010.81746134-35
GGCGAAT50200.010.76693251
CTCGGAT59500.010.7428581
Produced by FastQC (version 0.11.5)