FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22918080
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG110000.036.3609194
CAAGTCG84900.021.53474894
TAAGTCG27800.018.0899394
GAAGTCG107250.017.04708794
GTCCGAT15800.016.360761
GTTCGAT28600.013.9685321
CGCTTAA43550.012.842710544-45
TTAGCGA23300.012.5064388
CGCGTAT4608.033712E-712.260871
TAGCGAG27950.011.771029
CGTATCG22050.011.7233563
CGATTGG19950.011.3082714
TTCGATT35050.010.8616272
AAAGTCG48050.010.85744194
CGGGAAT52850.010.31598951
TATACGG19650.010.28498734-35
CGGAAAT44500.010.2449441
GTTTATT120450.010.184311
CACGCTT56800.010.05369842-43
CCGTATC32950.09.9848252
Produced by FastQC (version 0.11.5)