FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23492950
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG122500.052.79346594
CAAGTCG91800.025.13834494
TAAGTCG37300.021.9249394
GAAGTCG134600.019.51931694
GTCGGTT45150.013.6367651
GTTTTTT301000.012.2262451
CGAAGAT87500.012.1931411
CGGAAAT49000.011.9897961
GGAAAAT200250.011.4067411
AAAGTCG54850.011.22515994
CGCGGAT23400.011.04700851
GTTTAAT101850.010.9828181
CGCGTTT15750.010.7428571
CGCAAAT30850.010.6645061
GTTTATT117350.010.65361
TATACGC11050.010.6334847
GTTTTTC189200.010.55761051
CTTTAAT117750.010.4976641
TCGGATC47000.010.3999992
TACGCTA10003.6379788E-1210.3399999
Produced by FastQC (version 0.11.5)