FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23492950
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG149050.043.7363394
CAAGTCG108200.023.49999894
TAAGTCG42150.020.40569394
GAAGTCG131750.017.15901494
CGCGTAT5209.094947E-1114.4615371
CGAAAAT41950.013.3325381
TATACGC8700.012.9655167
CGCGTTT16100.012.5527941
AAAGTCG60500.012.42975194
CTCGTTT44050.012.0567541
CGAAGAT81250.012.0321
CGCAATT25700.011.8871591
CTTTAAT116350.011.8762351
CTCAGAT176100.011.7700171
GTTTAAT89950.011.1295171
GTTTTAT150850.010.9360281
CGGAAAT46750.010.7572191
GTTTTTT293100.010.7437731
CGCGGAT27450.010.61566451
ATATACG12450.010.5702816
Produced by FastQC (version 0.11.5)