FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005305997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005305997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22222580
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAT28750.017.6556511
TACGCTA20000.017.1559
TATACGC19250.017.0909087
TTTTTTC447300.016.3916822
CTTAGAT119100.016.337531
TAAGCAT73250.016.105128
TTTTTTG436550.016.0739882
GTTTAAT122650.016.0562571
AAGTCGG69900.016.00286194
CGGACAT59500.015.877311
ATATACG22800.015.6666656
AAGCATA76250.015.5331159
GTTTTTT311450.015.1510671
CTCGATT68800.014.8924421
GTTTTAC75850.014.4377041
ATTAAGC67950.014.3178816
GAATTTA88400.014.1425333
CGAAAAC58600.013.9556311
CTGAATT115800.013.7996551
TTTTTTA425900.013.7722462
Produced by FastQC (version 0.11.5)