FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306000

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306000
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22789854
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG141350.042.1620194
CAAGTCG109350.023.33882194
TAAGTCG33300.023.28828894
GAAGTCG129400.018.99613894
AAAGTCG59400.014.71717294
CGAATCG15250.012.6360663
CGAAAAT38500.012.5740261
TATACGC9400.012.5000017
CGAAGAT83350.011.7288541
GTCGGTT43850.011.4686431
CGCAATT22300.011.3811671
CGGAAAT43650.011.3058411
GGAAAAT180050.011.015831
AAATCCA200650.010.938954
GTTTTTT279250.010.8895261
GTTTAAT87400.010.8627011
TTAGCGA18300.010.7868857
CGCAAAC40800.010.598041
CGCAAAT35500.010.4591551
AATCGGG41900.010.4319815
Produced by FastQC (version 0.11.5)