FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22770912
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG140550.049.72536594
CAAGTCG119350.029.3774694
TAAGTCG32600.026.23926494
GAAGTCG128650.020.27594294
GTTCGAT30350.014.8665571
GTCCGAT15550.013.9035371
TTCGATT33700.011.7151352
CGCGTAT4951.9456038E-611.393941
CTCGGTT36150.010.7911481
CGCGTAA6554.2826287E-810.7633591
AAAGTCG47200.010.75423794
CGCGTGT24600.010.6991881
TATACGG16050.010.54205634-35
GTCGTTT24800.010.4233881
TTAGCGA23750.010.2905278
CGCGGAT25700.010.2412451
GTCGGTT48550.09.5839341
CGGGAAT53050.09.5683331
TAGCGAG28650.09.5148349
GGCGATT42500.09.5105891
Produced by FastQC (version 0.11.5)