FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306003

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306003
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22054099
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG83650.046.5224294
CAAGTCG66800.022.4446194
TAAGTCG27150.018.52302294
GAAGTCG104700.017.05826494
GTTTTTT295250.016.2530061
TACGCTA9050.015.0607749
GCGAAAT27900.014.6559151
GTTTAAT98350.013.5241491
GTCGATT20150.013.2952861
CGAATCG17550.013.1225083
GAAAAAT240450.013.0376391
CGCAAAC40300.012.945411
GTTTTAC76050.012.9165021
TTTTTTC376150.012.8573712
CGCAAAT33550.012.7481371
CTCGATT47750.012.6973831
CGAAAAT36500.012.4904121
AAATCCA199000.012.375884
GTTTTTC174950.012.2772231
GTTTTAT165550.012.2645731
Produced by FastQC (version 0.11.5)