FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306004

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306004
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22054099
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG113300.034.3477594
CAAGTCG79300.020.21059494
TAAGTCG31200.018.67948794
TATACGC11400.016.491237
CGAAAAC38900.015.4652971
GAAGTCG106750.015.01358494
GTTTTTT289700.014.6499821
TACGCTA11450.014.3668139
ATATACG13500.014.27407556
CTCGATT44450.013.6400461
CGCGAAC17050.013.5073321
CGAAAAT44900.013.5033411
CGCGTTT15450.013.0809071
CGCGTAT5051.346416E-813.0297041
GCGAAAT26450.012.7939511
AAATCGA32200.012.5527964
GTTTAAT93900.012.3631531
CTTAGAT72900.012.1851851
CGAAGAT82600.012.1767551
GTCGAAT13550.012.1402221
Produced by FastQC (version 0.11.5)