FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306005

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306005
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22035150
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG63500.036.34173294
TAAGTCG19050.018.99737494
GTTTATT114450.016.2210561
CAAGTCG50150.014.80757794
GTCGGTT57600.014.1979161
GTTCGAT27650.012.9186251
CGCGTAA4102.9745097E-612.60975551
AATCGGG55750.012.2242155
GTTTAAT107850.012.2021321
GTTTTAT169650.012.0512821
GGAAAAT182800.012.0328221
GTTTTCT240550.011.8989811
GTTTTTG178550.011.3979281
GTTGATT84350.011.3669221
GCGGTAT21250.011.2799991
GTTTTTT330900.011.135691
TCGATTC30050.011.1048242
GTTTGAT136200.011.0770921
GTCAATT60850.011.0451931
CGCGGGT60550.010.9446741
Produced by FastQC (version 0.11.5)