FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306006

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306006
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22035150
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG86700.027.05074794
CAAGTCG65800.013.42857194
GTTTATT123250.013.0036511
GTCGTTT20650.012.7457621
CGCAAAT28150.012.3552391
GTTCGAT30650.012.2675361
GTAAAAC70750.012.0904591
CGCGTAT5858.070856E-912.0512821
CGCAATT21300.011.9154921
CGCAAAC43650.011.7365411
CGCTAAT9800.011.5102021
GTTTTAT155300.011.4700571
CTCGTTT36150.011.4412161
CGAAAAT52700.011.4155591
CTTTATT174200.011.4127441
GGAAAAT159950.011.3422941
GTTTAAT100350.011.1469851
TAAGTCG21550.011.1229794
CGAATCG15050.010.9302323
CAATCGG34850.010.9239594
Produced by FastQC (version 0.11.5)