FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306008

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306008
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22051026
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG159650.050.4591394
TAAGTCG35250.028.93333294
CAAGTCG123250.027.99026594
GAAGTCG142600.022.37938594
AAAGTCG60100.016.42262894
GTTTTTT271500.015.2511981
CGAAAAC31150.012.5232751
GTCGGAT37800.012.43386394
GTTTAAT78100.012.096031
CGCGTTT16000.012.043751
GTTTTCT205400.011.3266791
CTCGCAT54650.011.0942351
AAATCGA30150.011.0679934
CGCAAAC46800.011.04700851
CTCGTTT35600.010.9578661
GCGAAAT28750.010.9530441
AAATCCA193550.010.9031264
GGAAAAC144200.010.886271
AATCCAC113050.010.850955
CGGAAAT44200.010.8461541
Produced by FastQC (version 0.11.5)