FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306009

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306009
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21570968
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG171500.056.92069694
CAAGTCG125950.030.22627894
TAAGTCG41850.028.4133894
GAAGTCG165800.026.39143694
AAAGTCG53100.018.41054594
TATACGG14600.011.9109588
GTATACG16400.010.6036587
TATATCG8803.1832315E-1010.1477277
TACGATT16800.010.07142894
ACAAGTC124400.09.89871392-93
CGATAAT31550.09.5340734
TTACGAG27400.09.4343061
CGAAGTC78750.09.13142894
ATAAGTC52800.09.07954594
GCAAGTC117250.09.03923292-93
ACGGAAC42800.08.8948593
AGAAGTC241750.08.74870794
TTAAGTC62900.08.74244994
GTAGTGA63850.08.6123716
TATGAGT57400.08.5156795
Produced by FastQC (version 0.11.5)