FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306012

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306012
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22947075
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG151500.047.86864594
CAAGTCG116050.026.4058694
TAAGTCG35250.023.06666894
GAAGTCG142900.020.22743294
AAAGTCG62650.015.07901194
TATACGC7350.014.0680287
CGAAAAT42600.012.2464791
CGCGTTT14700.012.1496611
CGCGAAC17950.011.5208911
CGGAAAT46450.011.1302481
GGCGAAT37250.010.9771821
GTCGGTT41200.010.9514571
CGAAGAT84900.010.7396941
TACGCTA7502.5484042E-910.6533349
ATATACG11050.010.6334856
GTTTTTT273400.010.555231
GGAAAAT196100.010.5456411
GTTTAAT85250.010.4750751
CTCAGAT172350.010.4444461
GGAAAAC163300.010.3325181
Produced by FastQC (version 0.11.5)