FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22478822
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG118850.061.05847594
CAAGTCG98700.032.094
TAAGTCG32050.027.12948494
GAAGTCG126500.022.03241294
AAAGTCG47850.015.42110794
CGTTCGA18600.013.1397867
CGCGTAT5353.014611E-812.2990671
ACGTTCG24250.012.0164956
AATCGTA22700.011.5947135
GTCGAAA35700.011.1904761
GTCGGGT43850.011.1470921
GCCGGTT51450.011.053451
TTAGCGA19800.010.9191928
GCGGTAT23250.010.9161291
GTCGAAT14350.010.8083631
GTTCGAT29150.010.8027451
GTCCGAT14450.010.7335631
ATCGTAT19750.010.7088616
GTCGATT21550.010.6867751
CGCTTAA34800.010.60201144-45
Produced by FastQC (version 0.11.5)