FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306018

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306018
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20867317
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGATCG30350.017.34431694
CGAAAAC35750.015.118881
GTCGAAT11850.014.2784811
GTTTTTT304800.014.2480311
CGCGAAT5753.092282E-1113.8956531
CGAAAAT39250.013.8904461
CTCGATT46750.013.8737971
AAATCGA25800.013.8449614
GCGAAAT24900.013.40160751
CGGAAAT36600.013.098361
CGAAGAT72350.013.0573611
TCAACGC44700.012.617456
CAACGCA48700.012.5462027
AAATCCA181800.012.5126514
AAAAATC192100.012.3800112
CGCAAAC37700.012.2175061
CTCAGAT155150.012.1475991
AATCCAA96050.011.9885475
CTTAGAT74950.011.8519011
ATCAACG47600.011.8487415
Produced by FastQC (version 0.11.5)