FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21687204
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG108850.051.81442394
CAAGTCG83550.025.14542294
TAAGTCG30200.023.6556394
GAAGTCG127200.019.43553494
TATACGC8350.018.0119787
AAAGTCG42950.017.39930294
GTTTTTT243800.016.7912221
TTTTTTC314800.016.3335442
CGCGAAT16000.015.8625011
TACGCTA10100.014.89108859
GCCGAAT51100.014.7162431
GAAAAAT177300.014.1556681
AAAAATC180450.013.4397342
GTTTAAT71550.013.3347311
GTTTTAC56400.013.251
GGAAAAT140800.013.1519891
CGAATTG29100.012.9209613
TTTTTTG345600.012.8651622
CTCGATT37250.012.7436251
ATATACG12950.012.70270256
Produced by FastQC (version 0.11.5)