FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21687204
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG137950.041.46357394
CAAGTCG102600.024.59941594
TAAGTCG30800.020.60064994
GAAGTCG125450.017.9083394
CGCGAAT13900.017.2446041
CGCGTAT3454.2561987E-714.9855071
GTTTTTT253800.014.6111111
GCGAAAT28750.013.5686961
AAAAATC163250.012.6101072
TACGCTA8250.012.5333349
GGAAAAT144050.012.2353341
AAAGTCG54850.012.1677394
AAATCCA179250.012.1138084
CGCGAAC9700.012.1134021
AAATCGA30750.012.0747974
CGAAAAC31950.012.0625971
CGAAAAT38600.011.9326431
AATCCAA88850.011.7433885
CTCGATT37800.011.6878311
CGCAAAC43200.011.5324081
Produced by FastQC (version 0.11.5)