FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306023

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306023
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22986286
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG91450.048.87589394
CAAGTCG72800.023.11263794
TAAGTCG28850.022.31889294
GAAGTCG112000.017.1214394
GTTCGAT28200.014.3333351
AAAGTCG41800.013.26794394
GTCGGTT39300.011.0025441
TTCGATT33050.010.9500772
CTCGGTT42300.010.8888891
CTCGGAT61500.010.2406511
TCGGATT27050.010.0776342
CGGAAAT43600.010.025231
TATACGG15200.09.74013234-35
GTTTATT118000.09.7186441
CGTTCGA15500.09.7032277
GGCGATT39900.09.6591481
GTCGAAT14650.09.6245741
GTCGTAT10851.6370905E-119.5299541
GCGGTAT21550.09.3781911
GTGAATT119200.09.3053691
Produced by FastQC (version 0.11.5)