FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306025

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306025
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22582463
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG102150.051.62408494
CAAGTCG81050.027.31277394
TAAGTCG28750.025.01217594
GAAGTCG119850.019.60784594
CGCGTAT5059.0039975E-1013.9603961
AAAGTCG47200.013.54237494
GTCCGAT16350.013.5107041
GTTCGAT30600.012.2875811
GTCGTTT20500.011.4634151
AATCGTA21550.011.1229715
CTCGGTT36350.011.1196711
GTCGGTT45500.010.4329671
TTAGCGA21750.010.15632258
CGGGAAT51450.010.1399421
GGCGAAT50000.010.0580011
GTTTATT102600.09.9405461
GTCGAAT12500.09.7760011
CGTAACG5305.123477E-59.7547173
CGCGTAA10609.094947E-129.7547171
TCGGATA21250.09.510592
Produced by FastQC (version 0.11.5)