FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22582463
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG128650.040.73455494
CAAGTCG94550.026.9423694
TAAGTCG30100.021.86046694
GAAGTCG124050.016.8222594
GTTCGAT31750.012.730711
GCGGTAT23800.012.6386551
CGCGAAT10000.012.2200011
CGTATCG19200.011.753
GTTTATT109550.011.1118211
AAAGTCG51550.010.94083494
TTCGATT35750.010.9118882
CGGGAAT52300.010.6940741
GTCGTAT10650.010.591551
AATCGTA21300.010.591555
ATCGTAT19150.09.8172326
TATACGG17750.09.79718334-35
CGCGTAT4359.004416E-49.7241381
CCGTATC28300.09.6325092
GGGAAAT153350.09.3785461
TAATCGT22100.09.3574674
Produced by FastQC (version 0.11.5)