FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306027

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306027
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22277863
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG99000.052.74444694
CAAGTCG78950.026.72957894
TAAGTCG31700.022.8328194
GAAGTCG113000.018.25929394
CGAATCG15700.014.9681533
CGGACAT56600.012.6219081
CGGAAAT53800.011.9684021
AAAGTCG57200.011.83216994
CTCGGAT73700.011.7340571
AATCGGG38900.011.5989725
AATCGTA23600.011.5508495
GTCGATT18350.011.5258871
CGAGAAC67250.011.461711
GTTTATT125200.011.3370621
TCGGGTC57800.011.3027682
CGCAAAT29400.011.1904771
GTTTAAT100900.011.1793861
GGCGAAT38200.011.0732991
GTTTTTT287700.010.8637481
TCGGATC47500.010.5873692
Produced by FastQC (version 0.11.5)