FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21537747
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG189700.060.4285794
CAAGTCG135850.031.34486694
TAAGTCG48150.028.89304494
GAAGTCG177700.027.11029894
AAAGTCG59300.019.18043794
TATACGG16450.013.1428588
TACGATT17150.012.05830994
GTATACG18200.011.8791217
CGCGTAA8700.011.07471488-89
TTACGAG31400.010.9267511
ACAAGTC129350.010.90065894
CGTATCG18250.010.81643836-37
ATAAGTC54550.010.68377692-93
CGCGTTA4551.1860762E-410.329677
GCAAGTC120000.010.26166692-93
TAGCGTA8702.8067006E-99.7241384
CGATAAT41900.09.6467774
CGAAGTC84700.09.62750992-93
AGAAGTC248300.09.31292894
TATATCG8652.6375346E-89.2369957
Produced by FastQC (version 0.11.5)