FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306030

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306030
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21537747
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTTTT282400.016.5099141
CTCGATT37200.015.5403211
GCGAAAT28050.013.739751
CGAAAAC33500.013.46865751
CGAAAAT45250.013.3988941
AAATCCA183200.013.0070964
CGCAAAC42350.012.8736721
CGGATAT20900.012.8181821
GGAAAAT164550.012.5676081
AAAAATC190550.012.3820522
CGCATAT9150.012.3278691
TACGCTA7701.8189894E-1212.2077939
CTTAGAT50100.012.1956091
CTTTAAC74050.012.1863611
GTTTAAT87300.012.1672391
TCGATTG21100.012.0284362
CGCGTAT4355.763235E-611.8850581
CTCAGAT165850.011.8739831
GGAAAAC140600.011.8669991
CGCAATC22300.011.80269051
Produced by FastQC (version 0.11.5)