FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306033

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306033
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23016708
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG100700.049.19364594
CAAGTCG73400.025.54904694
TAAGTCG29500.022.30508494
GAAGTCG114700.017.9476994
GTTTTTT306050.017.2151581
TTTTTTC416450.016.5450842
TATACGC7900.016.0632927
CGAAAAT44050.015.3643591
TTTTTTG458450.014.9985822
GTTTAAT111650.014.859831
GTTTTAC69300.013.971141
CTCGATT44750.013.86368751
AAAGTCG48600.013.73251194
CGAAAAC36300.013.7245181
TACGCTA8950.013.6536319
CGCAAAC51450.013.6112731
TTTTTTA437700.013.6049812
GTTTTCT226200.013.4018571
AATCCAC122650.012.9906245
GGAAAAT184150.012.9655171
Produced by FastQC (version 0.11.5)