FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23016708
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG123300.040.0243394
TAAGTCG33700.021.75667694
CAAGTCG90150.020.80199694
GAAGTCG113900.017.00087794
GTTTTTT337600.015.81516551
CGAAAAT47600.014.2184871
CTCGATT48100.014.16841
CGCAATT21700.013.8617521
GCGAAAT27900.013.8136211
CGCAAAC42050.013.6361471
TATACGC10900.013.3669737
CTTAGAT62250.013.1373491
TACGCTA10450.012.5933029
CTCAGAT173150.012.5677171
AAAGTCG52300.012.49139694
AAAAATC214450.012.3609242
CTTTAAT116300.012.3258811
GTTTAAT104450.012.2843471
CTCGGAT68000.012.1647051
CGAAAAC38050.012.1051251
Produced by FastQC (version 0.11.5)