FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005306038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005306038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22983676
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG117150.047.06018494
CAAGTCG95350.027.89932394
TAAGTCG30600.021.5032794
AATCGTA22350.017.454145
GAAGTCG121150.016.6042194
GTTCGAT26400.014.4204551
ATCGTAT17850.013.6918766
CGCTTAA35400.012.87853244-45
AAAGTCG49350.011.80952594
TTCGATT29950.011.455762
TCGTATC19300.010.95854957
GCGGTAT22500.010.8622231
CGGTATA14100.010.6666672
GCCGGTT48500.010.5628861
ATTAGCG10153.6379788E-1210.1871926
GTCGGTA17100.010.1695911
TTAGCGA18850.09.97347458
GGCGAAT36900.09.934961
GTCGTTT22300.09.9058291
GTCCGAT16300.09.8036811
Produced by FastQC (version 0.11.5)